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human recombinant tgf β3 protein  (MedChemExpress)


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    Structured Review

    MedChemExpress human recombinant tgf β3 protein
    Human Recombinant Tgf β3 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+tgf+%CE%B23+protein/TGF+beta+3%2FTGFB3%2C+Human/pmc13006418-469-93-100
    Average 94 stars, based on 4 article reviews
    human recombinant tgf β3 protein - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: A Self-Assembling Immune-Featured Osteosarcoma Patient/PDX Derived Organoid Model and Biobank for Personalized Immune Therapy
    Article Snippet: OS-Dissolver and OS-Terminator were prepared in SeekGene ( SeekGene Corporation , China). .. Each 50 ml of OS-Nurturer was composed of penicillin-streptomycin solution (1%), FBS (10%), GlutaMAX TM supplement (1%, #35050061, Gibco ), N-2 supplement (1.2%, #17502048, ThermoFisher ), MEM Non-Essential Amino Acids (NEAA) (1.2%, #11140050, ThermoFisher ), B-27 TM supplement (1.2%, #17504044, ThermoFisher ), Insulin, Transferrin, Selenium Solution (ITS-G) (1.2%, #41400045, Gibco ), Ascorbic acid (100 μmol/L, #200-066-2, Merck ), ROCK inhibitor Y-27632 (10 μmol/L, #10035-04-8, Merck), human recombinant EGF protein (20 ng/mL, #AF-100-15, PeproTech ), human recombinant bFGF protein (40 ng/mL, #HY-P7331A, MedChemExpress ), human recombinant IGF-1 protein (20 ng/mL, # HY-P70783, MedChemExpress ), human recombinant TGF-β3 protein (10 ng/mL, #HY-P7120, MedChemExpress ), Adenine (188 μmol/L, #HY-B0152, MedChemExpress ), ALK inhibitor A 83-01 (0.5 μmol/L, #HY-10432, MedChemExpress ), selective p38 MAPK inhibitor SB 202190 (10 μmol/L, #HY-10295, MedChemExpress ), Dexamethasone (10 - mol/L, #HY-14648, MedChemExpress ), human recombinant Wnt3a protein (500 ng/ml, #HY-P70453A, MedChemExpress ) and OS-Nurturer was then brought up to 50 ml with DMEM. .. OS-Nurturer was sterilized by filtration using a 0.22 μm syringe filter (#SLGP033RS, Millipore ).

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy
    Article Snippet: Human recombinant IGF-1 protein , MedChemExpress , Cat#HY- P70783. .. Human recombinant TGF-β3 protein , MedChemExpress , CatHY-P7120. .. Adenine , MedChemExpress , Cat#HY-B0152.

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: Both the dissociation solution and dissociation termination solution were prepared by SeekGene Corporation (SeekGene Corporation, China) under standardized protocols to ensure consistency and reproducibility. .. Organoid culture medium and cryopreservation solution preparation The culture medium was formulated by combining the following components to a final volume of 50 mL: penicillin-streptomycin solution (1%), FBS (10%), GlutaMAX supplement (1%, #35050061, Gibco), N-2 supplement (1.2%, #17502048, ThermoFisher), MEM Non-Essential Amino Acids (NEAA) (1.2%, #11140050, ThermoFisher), B-27 supplement (1.2%, #17504044, ThermoFisher), Insulin, Transferrin, Selenium Solution (ITS-G) (1.2%, #41400045, Gibco), ascorbic acid (100 μmol/L, #200-066-2, Merck), ROCK inhibitor Y-27632 (10 μmol/L, #10035-04-8, Merck), human recombinant EGF protein (20 ng/mL, #AF-100-15, PeproTech), human recombinant bFGF protein (40 ng/mL, #HY-P7331A, MedChemExpress), human recombinant IGF-1 protein (20 ng/mL, #HY-P70783, MedChemExpress), human recombinant TGF-β3 protein (10 ng/mL, #HY-P7120, MedChemExpress), adenine (188 μmol/L, #HYB0152, MedChemExpress), ALK inhibitor A 83-01 (0.5 μmol/L, #HY-10432, MedChemExpress), selective p38 MAPK inhibitor SB 202190 (10 μmol/L, #HY-10295, MedChemExpress), dexamethasone (10 ̂-5 mol/L, #HY-14648, MedChemExpress), and human recombinant Wnt3a protein (500 ng/mL, #HY-P70453A, MedChemExpress). .. The mixture was adjusted to 50 mL with DMEM and sterilized by filtration through a 0.22 μm syringe filter (#SLGP033RS, Millipore).

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy
    Article Snippet: Both the dissociation solution and dissociation termination solution were prepared by SeekGene Corporation (SeekGene Corporation, China) under standardized protocols to ensure consistency and reproducibility. .. The culture medium was formulated by combining the following components to a final volume of 50 mL: penicillin-streptomycin solution (1%), FBS (10%), GlutaMAX supplement (1%, #35050061, Gibco), N -2 supplement (1.2%, #17502048, ThermoFisher), MEM Non-Essential Amino Acids (NEAA) (1.2%, #11140050, ThermoFisher), B-27 supplement (1.2%, #17504044, ThermoFisher), Insulin, Transferrin, Selenium Solution (ITS-G) (1.2%, #41400045, Gibco), ascorbic acid (100 μmol/L, #200-066-2, Merck), ROCK inhibitor Y-27632 (10 μmol/L, #10035-04-8, Merck), human recombinant EGF protein (20 ng/mL, #AF-100-15, PeproTech), human recombinant bFGF protein (40 ng/mL, #HY-P7331A, MedChemExpress), human recombinant IGF-1 protein (20 ng/mL, #HY- P70783 , MedChemExpress), human recombinant TGF-β3 protein (10 ng/mL, #HY-P7120, MedChemExpress), adenine (188 μmol/L, #HY-B0152, MedChemExpress), ALK inhibitor A 83-01 (0.5 μmol/L, #HY-10432, MedChemExpress), selective p38 MAPK inhibitor SB 202190 (10 μmol/L, #HY-10295, MedChemExpress), dexamethasone (10ˆ-5 mol/L, #HY-14648, MedChemExpress), and human recombinant Wnt3a protein (500 ng/mL, #HY-P70453A, MedChemExpress). .. The mixture was adjusted to 50 mL with DMEM and sterilized by filtration through a 0.22 μm syringe filter (#SLGP033RS, Millipore).



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    A. Schematic representation of GBA1 and GBAP1 . B. gRNA specifically targets GBA1 to generate the KO line. C. Editing outcome in the KO line revealed by short-read amplicon sequencing. D. Reversion to the WT line using CRISPR-Cas9 and piggyBac transposase. E. Editing outcome in the WT line revealed by short-read amplicon sequencing. F . Specific editing of GBA1 as demonstrated by PacBio HiFi long-read amplicon sequencing.
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    A. Schematic representation of GBA1 and GBAP1 . B. gRNA specifically targets GBA1 to generate the KO line. C. Editing outcome in the KO line revealed by short-read amplicon sequencing. D. Reversion to the WT line using CRISPR-Cas9 and piggyBac transposase. E. Editing outcome in the WT line revealed by short-read amplicon sequencing. F . Specific editing of GBA1 as demonstrated by PacBio HiFi long-read amplicon sequencing.
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    Image Search Results


    A. Schematic representation of GBA1 and GBAP1 . B. gRNA specifically targets GBA1 to generate the KO line. C. Editing outcome in the KO line revealed by short-read amplicon sequencing. D. Reversion to the WT line using CRISPR-Cas9 and piggyBac transposase. E. Editing outcome in the WT line revealed by short-read amplicon sequencing. F . Specific editing of GBA1 as demonstrated by PacBio HiFi long-read amplicon sequencing.

    Journal: bioRxiv

    Article Title: Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by β-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonism

    doi: 10.1101/2025.06.23.661126

    Figure Lengend Snippet: A. Schematic representation of GBA1 and GBAP1 . B. gRNA specifically targets GBA1 to generate the KO line. C. Editing outcome in the KO line revealed by short-read amplicon sequencing. D. Reversion to the WT line using CRISPR-Cas9 and piggyBac transposase. E. Editing outcome in the WT line revealed by short-read amplicon sequencing. F . Specific editing of GBA1 as demonstrated by PacBio HiFi long-read amplicon sequencing.

    Article Snippet: On day 10, media was changed to Neurobasal/GlutaMAX/B-27 with 20 ng/ml brain-derived neurotrophic factor (BDNF, Peprotech, 450-02), 20 ng/ml glial cell line-derived neurotrophic factor (GDNF, Peprotech, 450-10), 200 μM ascorbic acid (Sigma-Aldrich, 4034), 1 ng/ml transforming growth factor type β3 (TGFβ3, R&D Systems, 243-B3), 200 μM dibutyryl cAMP (Sigma-Aldrich, D0627) and 3 μM CHIR99021.

    Techniques: Amplification, Sequencing, CRISPR